Preparation of a paeonol-containing temperature-sensitive in situ gel and its preliminary efficacy on allergic rhinitis.

In this paper, the optimal composition of Paeonol temperature-sensitive in situ gel is composed of poloxamer 407 (P407) are determined, and preliminary studies conducted its effect on allergic rhinitis. Paeonol optimum composition of the temperature-sensitive in situ gel including 2% Paeonol inclusion, 22% P407, 2% poloxamer 188 (P188) and 2% PEG6000, as assessed by the study of thermodynamic and rheological. Model frog palate was employed to study  Preparation of a phenol-containing temperature-sensitive in situ gel and its preliminary efficacy on allergic rhinitis. Cavia Clia Kits the toxicity of Paeonol temperature-sensitive in situ gel in the nasal mucosa. The results showed low toxicity to the cilia, which allows the gel to be used for nasal administration. 

Franz diffusion cell method is used to study the in vitro release of Paeonol and suggests that the in vitro release is in line with Higuchi equation. These results indicate that Paeonol can be absorbed into the body through the mucous membrane and has some characteristics of a sustained effect. Finally, the guinea pig model of allergic rhinitis sensitized to ovalbumin were used to evaluate the initial effectiveness of the gel, with the temperature-sensitive in situ gel Paeonol show significant effects on sensitized guinea pig model of allergic rhinitis (AR).

The cilia beat frequency (CBF) was measured in the fallopian tube fimbria slice preparation, use videomicroscopy at high speed (500 Hz) cameras in mice treated with β-estradiol benzoate (βE2B) and Medroxy progesterone (mPRG). In non-ovulation guinea pig at 4 weeks of age, the CBF of high fimbria (17.8 Hz). In the sexually mature guinea pigs (12-16 weeks of age) with constant ovulation, CBF varies from 12 Hz to 16 Hz. 

The in vivo administration of both ICI-182 780 (βE2 receptor blocker) and mifepristone (PRG receptor blocker) induced high CBF (17.4 Hz). Giving βE2B at a low (3.2 mg / kg / day) or high (32 mg / kg / day) dose of the CBF decreased to 14.5 Hz or 11 Hz, respectively. ICI-182 780 βE2B eliminate change-induced decrease in CBF and CBF to 12 Hz. MPRG administration (6.4 mg / kg / day) decreased  https://gentaur.fr/ CBF to 12.5 Hz. Mifepristone is abolished mPRG-induced decrease in CBF and CBF is maintained at 15 Hz. However, the provision of both βE2B and mPRG increased CBF to 17.5 Hz, indicating that inhibiting the action βE2B mPRG and vice versa. 

To confirm the interaction between βE2B and mPRG, we were given both βE2B and mPRG for guinea pigs pre-treatment for 1.5 days by both mPRG (6.4 mg / kg / day) or βE2B (3.2 mg / kg / day ). previous treatment with βE2B or mPRG prevent an increase in CBF stated by βE2B plus mPRG and CBF maintained at 14.5 Hz or 13 Hz, respectively.